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Plasmid alkaline lysis precipitation

18 listings from 10 suppliers · 4 Japanese makers

Japanese name: アルカリ溶菌・沈殿用試薬(NaOH/SDS・CaCl2)

Alkaline lysis and precipitation reagents, meaning NaOH/SDS lysis solutions and neutralization or selective precipitation buffers, start the primary recovery of plasmid from bacterial cells. They denature genomic DNA and release the plasmid. Buyers choose between ready-made solutions matched to a purification kit series for research and pharmaceutical-grade NaOH and SDS raw materials for GMP. They also compare lysis-completion indicator dyes, supply volume and local sourcing. What follows is our list of tracked products, with makers and the Japanese companies that sell them.

Neutralization and precipitation buffers (for primary recovery)
ProductMakerWhere to buy in Japan
Buffer P3 (Neutralization Buffer)QIAGENNot yet confirmed
NucleoBond Neutralization Buffer S3Macherey-NagelNot yet confirmed
Neutralization Solution (PureYield)PromegaNot yet confirmed
GMP / pharmaceutical-grade raw materials (NaOH, SDS)
ProductMakerWhere to buy in Japan
Sodium hydroxide solution EMPROVE EXPERT (1M / 50%)MerckMerck Ltd., Japan (Japanese subsidiary, Life Science)
Sodium hydroxide pellets EMPROVE ESSENTIALMerckMerck Ltd., Japan (Japanese subsidiary, Life Science)
Sodium dodecyl sulfate EMPROVE ESSENTIAL (Ph Eur)MerckMerck Ltd., Japan (Japanese subsidiary, Life Science)
Sodium Hydroxide 10N Solution, Biotech ReagentAvantorNot yet confirmed
Sodium Hydroxide 10N Solution, WFI Quality WaterAvantorNot yet confirmed
Bulk research reagents (Japanese makers)
ProductMakerWhere to buy in Japan
Sodium dodecyl sulfate (SDS, 151-21-3)FUJIFILM WakoJP makerFrom the maker
Sodium n-dodecyl sulfate (Cica)Kanto ChemicalJP makerFrom the maker
Sodium hydroxide (Cica)Kanto ChemicalJP makerFrom the maker
10% SDS Solution (aqueous sodium dodecyl sulfate solution)Nippon GeneJP makerFrom the maker
Sodium lauryl sulfate (SDS)Nacalai TesqueJP makerFrom the maker

“Where to buy in Japan” shows only sales relationships we have confirmed on the company’s or the distributor’s own website. “Not yet confirmed” means we have not checked yet, not that the product is unavailable in Japan. Product names link to the maker’s own page.