Calculators for bioprocess and drug development
An answer in seconds, with what the number means, the formula behind it and the guidance it comes from. Each calculator also lists the Japanese terms you will meet in documents from Japan.
Draw
Design tools
Upstream
Cell culture
- Growth rate and doubling timeSpecific growth rate and doubling time from two viable cell density counts.
- Perfusion CSPR and medium useDaily medium volume, VVD and cost from a target CSPR and cell density.
- Scale-up agitation speedAgitation speed for a larger vessel at constant P/V, tip speed or Reynolds number.
- Titer to product massHarvest mass and final drug substance from titer, volume and recovery.
Downstream
Purification
- Residence time and linear velocityResidence time, linear velocity and load per cycle for a packed column.
- HETP and asymmetryCheck a packed column from a pulse injection.
- UF/DF concentration and diavolumesConcentration factor, buffer left after N diavolumes, and diavolumes needed.
- Step and overall yieldStep recovery from amounts in and out, and overall yield across four steps.
- TFF operating conditions (TMP, flux)Transmembrane pressure, flux in LMH and permeability from run data.
Formulation and preparation
Making and diluting solutions
Analytics and QC
Measuring quantity and quality
Nonclinical and DMPK
Evaluating drug candidates
- Human equivalent dose (HED)Convert an animal dose to a human starting dose with body-surface-area factors.
- Z′-factor (assay quality)Check how cleanly positive and negative controls separate before a screen.
- hERG safety marginhERG IC50 over free Cmax, read against the 30-fold benchmark.
- Caco-2 permeability (Papp)Apparent permeability and efflux ratio from transport data.
- Hepatic clearance from CLintScale a microsomal half-life to human hepatic clearance with the well-stirred model.
Every calculator gives an estimate for learning, built on published information and primary sources. Real decisions follow the applicable guidance and expert judgment. Look up other terms from Japanese documents in the Japanese–English glossary.