Restriction enzymes and reaction buffers
20 listings from 9 suppliers · 4 Japanese makers
Japanese name: 制限酵素・反応バッファー
Restriction enzymes cut DNA at specific sequences. In in vitro transcription (IVT) template preparation they linearize the plasmid, and Type IIS enzymes cut outside their recognition site so the template ends cleanly for defined-length RNA. Buyers compare use (linearization or plasmid identification), Type IIS availability, GMP grade and animal-origin-free status, star activity (nonspecific cutting), and universal buffer compatibility. The products we track are listed below, with the maker and Japanese seller of each.
| Product | Maker | Where to buy in Japan |
|---|---|---|
| BspQI (R0712) | New England Biolabs | Not yet confirmed |
| BsaI-HF v2 (R3733) | New England Biolabs | Not yet confirmed |
| SapI (R0569) | New England Biolabs | Not yet confirmed |
| BspQ I (for mRNA synthesis) | Takara BioJP maker | From the maker |
| GMP-Grade BsaI / BspQI | HZymes Biotech | Not yet confirmed |
| BspQI GMP-grade (10664ES) | Yeasen | Not yet confirmed |
| BsaI GMP-grade (10661ES) | Yeasen | Not yet confirmed |
| Product | Maker | Where to buy in Japan |
|---|---|---|
| BbsI-HF (R3539) | New England Biolabs | Not yet confirmed |
| FastDigest restriction enzymes | Thermo Fisher Scientific | Thermo Fisher Scientific K.K. (Japanese subsidiary) |
| Anza restriction enzyme cloning system | Thermo Fisher Scientific | Thermo Fisher Scientific K.K. (Japanese subsidiary) |
| Restriction Enzymes | Promega | Not yet confirmed |
| Restriction enzyme series (Pac I and others) | ToyoboJP maker | From the maker |
| EcoRI and other restriction enzymes | Nippon GeneJP maker | From the maker |
| Restriction enzymes / modifying enzymes | FUJIFILM WakoJP maker | From the maker |
| Product | Maker | Where to buy in Japan |
|---|---|---|
| rCutSmart Buffer (B6004) | New England Biolabs | Not yet confirmed |
| FastDigest Buffer / Green Buffer | Thermo Fisher Scientific | Thermo Fisher Scientific K.K. (Japanese subsidiary) |
| Buffer Set for Restriction Enzymes (B30) | Thermo Fisher Scientific | Thermo Fisher Scientific K.K. (Japanese subsidiary) |
| MULTI-CORE Buffer Pack | Promega | Not yet confirmed |
| Universal Buffer (for restriction enzymes) | Takara BioJP maker | From the maker |
| 10×RE Buffer Set | Nippon GeneJP maker | From the maker |
“Where to buy in Japan” shows only sales relationships we have confirmed on the company’s or the distributor’s own website. “Not yet confirmed” means we have not checked yet, not that the product is unavailable in Japan. Product names link to the maker’s own page.